Molecular Analysis in Forensic Science

Myrsini Voulgari

Co-Presenters: Maria Denise Gemmellaro

College: Hennings College of Science Mathematics and Technology

Department: Biological Sciences

Abstract:

This project explores the practical application of portable nanopore technology for the extraction and sequencing molecular DNA both from skeletal material and insects. Recovering DNA from degraded material continues to be one of the most persistent challenges in forensic science, particularly in cases where timely identification is critical. We are assessing the application of the Oxford Nanopore MinION Mk1D, a palm-sized device capable for real-time sequencing platform for this instances. DNA extraction and sequencing will be performed using a commercially available kit specifically designed for nanopore workflows. A key advantage of this platform is its portability, which allows sequencing to take place also in field environments. By creating protocols for both skeletal and insect-derived material, this project seeks to better understand its potential role in rapid, on-site forensic investigations. While nanopore sequencing has demonstrated promise in clinical and environmental genomics, its systematic evaluation for degraded skeletal remains and insects is still limited. The ability to generate rapid genetic profiles from compromised material could significantly enhance forensic response in time-sensitive investigations.