Cyclodextrin-coffee oil complex reduces lifespan and oxidative stress resistance of Caenorhabditis elegans

Eric Favaro

Co-Presenters: Yenil Arellano, Yaritza Cabrera

College: Hennings College of Science Mathematics and Technology

Major: BA.BIO/TCHRED

Faculty Research Mentor: Pereira, Renalison Farias  

Abstract:

Cyclodextrin-coffee oil complex reduces lifespan and oxidative stress resistance of Caenorhabditis elegansEric Favaro, Yenil Arellano, Yaritza Cabrera, and Dr. Renalison Farias-PereiraThe Dorothy and George Hennings College of Science, Mathematics and Technology, Kean UniversityCoffee and its derivative, coffee oil has shown to possess properties that may offer potentialhealth benefits, such as antioxidant effects. In this study, we examined the effects of coffee oilcomplexed with cyclodextrin on lifespan and oxidative stress resistance of Caenorhabditiselegans. Lifespan assays were performed using five treatment groups: vehicle control (0.4%DMSO), and 1 mg/mL of methyl-β-cyclodextrin, physical mixture, cyclodextrin-coffee oilcomplex, and coffee oil. A separate assay included paraquat, a known oxidative stress inducer,was used to further examine how the different treatments affected survival under oxidative stress.Worms treated with cyclodextrin had a higher probability of surviving shorter than wormstreated with only vehicle control. In contrast, coffee oil did not affect the lifespan of C. elegans.Further analyses of genes related to oxidative stress, such as SKN-1, will be performed usingfluorescence microscopy, enabling direct visualization and assessment of protein presence.Overall, the results suggest that cyclodextrin-coffee oil complex plays a role in oxidative stresspathways, but more experimentation is required.Keywords: animal model, antioxidant, biology, coffee, lifespan.