Effects of Caffeine on A549 Cell Proliferation

Marilyn Dennis

Co-Presenters: Jake Spring, Alexander Vanegas, Alejandro Ramirez, Yhaly Medelyn Gondim Reyes

College: Hennings College of Science Mathematics and Technology

Major: BS.BIO/CELL/MOLEC

Faculty Research Mentor: Thomas Comollo  

Abstract:

Caffeine is one of the most widely consumed stimulants worldwide that has been reported to enhance pathways that regulate cell survival and growth. The effects of caffeine on lung cancer cell proliferation remain unclear. This study investigates the impact of caffeine on the proliferation of A549 alveolar basal epithelial cells.The A549 cells are cultured and treated with different concentrations of caffeine over a specified experimental duration.The cell proliferation is evaluated by using a quantitative viability assay to determine metabolic activity which will be an indicator of cell growth. Data will be analyzed to determine whether exposure to caffeine will impact proliferation compared to the controls.This experimental study contributes to a better understanding of how dietary compounds can influence cancer cell growth, as well as highlight the importance of further research into the molecular mechanisms underlying caffeines effects. Future studies could further explore cell cycle progression and apoptotic markers.