The Acute Effects of Circuit Training and Traditional Resistance Exercise on Exerkines in Young Adults
Madhumitha Sadhasivan Gayathri
Co-Presenters: Zachariah Lowary
College: The Dorothy and George Hennings College of Science, Mathematics and Technology
Major: Biology
Faculty Research Mentor: Pragya Sharma Ghimire
Abstract:
Background: Irrefutable evidence supports that exercise is an essential component of healthy aging, and it has been widely accepted that a physically active life contributes to health-related quality of life. Many studies have quantified the influence of physical activity on bone health through longitudinal studies, cross-sectional studies, and meta-analyses of different populations. Many overlapping signaling pathways, including Wnt, Hedgehog, Growth Hormone (GH), Interleukin 6 (IL-6), Irisin, Tumor necrosis factor alpha (TNF-α), and receptor activator of NF-kB ligand (RANKL), serve as a fundamental mechanism for understanding muscle and bone metabolism as exerkines. Currently, the acute effects of exercise protocols on circulating exerkines are unclear. PURPOSE: This study aims to compare acute serum sclerostin, DKK-1, RANKL, TNF-α, Irisin, and IL-6 responses to circuit training and traditional resistance exercise. Methods: In this randomized crossover study, participants will perform two protocols separated by 2-week wash-out periods: 1. Circuit training (cycle ergometer, push-up, step-ups, medicine ball twist, and front squats with kettlebell for three sets) and 2. Traditional resistance exercise (3 sets 10 repetitions 80% 1RM for leg press, seated cable row, barbell bench press, kettle bell dead lifts, and dumbbell seated shoulder press. Fasting morning blood will be taken before exercise training (PRE), immediately post-exercise (IP), and 30 minutes post-exercise (30P). Using ELISA, blood samples will be analyzed for lactate, hematocrit, sclerostin, DKK-1, IL-6, TNF- α, RANKL, and irisin serum concentrations. Conclusion: Based on our observations about the acute bone turnover response to exercise and previous studies related to sclerostin and PTH, we hypothesized that circuit training would elicit a more significant release of circulating exerkines than traditional resistance exercises. Significance/Novelty: The results would contribute to better understanding how different exercise interventions can interact with the physiological systems associated with these exercises and bone remodeling. Recognizing the significance of these bone, muscle, and inflammatory markers aids in developing novel screening methods for predicting adverse health events in clinical and sports-performance populations. Additionally, these markers can be utilized to establish links with training regimens and monitor healthcare outcomes.Key Words: Exerkines, Physical Activity, and Inflammatory Markers