Identification and characterization of Polyporus fungi using molecular methods

Giselle Riera Torres

Co-Presenters: Omar Ahmed, Paul Oliveira, Kirelos Salib, Shahzaib Tahir, Aahana Shaahi

College: Hennings College of Science Mathematics and Technology

Major: BS.BIO/CELL/MOLEC

Faculty Research Mentor: Shumskaya, Maria  

Abstract:

Fungi includes over three million species that vary in shape, size, color, and habitat. As heterotrophic organisms, they obtain energy by excreting enzymes that break down organic matter, contributing to their ecological fulfillment. Fungi are more closely related to animals than plants and that are commonly found in moist forest environments, particularly on dead wood, play vital roles in nutrient cycling and supporting insect and animal ecosystems. Beyond their ecological importance, fungi have significant applications in medicine, pharmaceuticals, biotechnology, and nutrition. This study focuses on P. tremulae, a wood-decaying fungus found on aspen trees that is typically avoided by insects. The study investigates whether P. tremulae deters insects from its host tree, whether this effect is concentration-dependent, and whether the fungus synthesizes defensive compounds using nutrients from the tree. Laboratory-grown samples will be compared to natural specimens to evaluate metabolite concentrations and their role in insect deterrence or structural integrity. Our research The research aims to culture wood fungi in liquid and solid media, and confirm species identify using DNA barcoding method. The method includes isolation and amplify fungal DNA, followed by electrophoresis and sequencing, analysis of metabolites Of P. tremulae is conducted by mass spectrometry. This research is supported by the NSF grant to the principal investigator Dr. Shumskaya.